Organogenesis in meristems and cauline disc of Aloe vera L.
Aloe vera L. has been used as a medicinal plant and ornamental, so that this plant has acquired great commercial importance. However, A. veraL. has a reproductive disadvantage under natural conditions limiting supply of world demand. The objective of this study was evaluated process organogenesis de...
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| Format: | Online |
| Language: | spa |
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Universidad Pedagógica y Tecnológica de Colombia
2019
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| Online Access: | https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/9809 |
| _version_ | 1801706345626337280 |
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| author | Albarrán Ruiz, Yuri Pacheco Maldonado, José Constantino Rache Cardenal, Leidy Yanira |
| author_facet | Albarrán Ruiz, Yuri Pacheco Maldonado, José Constantino Rache Cardenal, Leidy Yanira |
| author_sort | Albarrán Ruiz, Yuri |
| collection | OJS |
| description | Aloe vera L. has been used as a medicinal plant and ornamental, so that this plant has acquired great commercial importance. However, A. veraL. has a reproductive disadvantage under natural conditions limiting supply of world demand. The objective of this study was evaluated process organogenesis development from meristematic tip and cauline discs. Meristematic tip and cauline discs were used as explants and cultured on Murashige and Skoog medium supplemented with different growth regulators. The percentage of organogenic and callogenic induction was quantified. Regenerated shoots were micropropagated on medium supplemented with 2 mg L-1BA and 0.1 mg L-1NAA, and rooting was tested under in vitroand ex vitroconditions.Highest percentage of callus formation in meristematic tip was quantified in presence of 0.5 and 1 mg L-12,4-D and 3 mg L-1NAA and in cauline disks in presence of 0.5 mg L-1of 2,4-D. During caulogenesis, highest average number of shoots per explant (1.1) was quantified from meristematic tip in presence of 5 mg L-1NAA, while in cauline disk (1.3 shoots/explant) were quantified in presence 0.25 mg L-12,4-D. After micropropagation cycle second, the average number of shoots per explant increased to 8.8. The highest percentage (75.3%) of rooted shoots was quantified in vitroconditions. In conclusion, the primary explant more desirable to initiate organogenic processes in A. verawas meristems cultured in medium with 5.0 mg L-1NAA. |
| format | Online |
| id | oai:oai.revistas.uptc.edu.co:article-9809 |
| institution | Revista Ciencia en Desarrollo |
| language | spa |
| publishDate | 2019 |
| publisher | Universidad Pedagógica y Tecnológica de Colombia |
| record_format | ojs |
| spelling | oai:oai.revistas.uptc.edu.co:article-98092020-11-11T02:06:30Z Organogenesis in meristems and cauline disc of Aloe vera L. Organogénesis a partir de ápices meristemáticos y discos caulinares de Aloe vera L. Albarrán Ruiz, Yuri Pacheco Maldonado, José Constantino Rache Cardenal, Leidy Yanira callos, cultivo in vitro, reguladores de crecimiento callus, in vitro culture, growth regulators Aloe vera L. has been used as a medicinal plant and ornamental, so that this plant has acquired great commercial importance. However, A. veraL. has a reproductive disadvantage under natural conditions limiting supply of world demand. The objective of this study was evaluated process organogenesis development from meristematic tip and cauline discs. Meristematic tip and cauline discs were used as explants and cultured on Murashige and Skoog medium supplemented with different growth regulators. The percentage of organogenic and callogenic induction was quantified. Regenerated shoots were micropropagated on medium supplemented with 2 mg L-1BA and 0.1 mg L-1NAA, and rooting was tested under in vitroand ex vitroconditions.Highest percentage of callus formation in meristematic tip was quantified in presence of 0.5 and 1 mg L-12,4-D and 3 mg L-1NAA and in cauline disks in presence of 0.5 mg L-1of 2,4-D. During caulogenesis, highest average number of shoots per explant (1.1) was quantified from meristematic tip in presence of 5 mg L-1NAA, while in cauline disk (1.3 shoots/explant) were quantified in presence 0.25 mg L-12,4-D. After micropropagation cycle second, the average number of shoots per explant increased to 8.8. The highest percentage (75.3%) of rooted shoots was quantified in vitroconditions. In conclusion, the primary explant more desirable to initiate organogenic processes in A. verawas meristems cultured in medium with 5.0 mg L-1NAA. Aloe veraL. ha sido utilizada como planta medicinal y ornamental, por lo que ha adquirido gran importancia comercial; sin embargo, presenta una desventaja reproductiva en condiciones naturales que limita el abastecimiento de la demanda mundial. El objetivo del estudio fue evaluar el desarrollo de procesos organogénicos a partir de ápices meristemáticos y discos caulinares. Se emplearon como explantes, ápices meristemáticos y discos caulinares cultivados en medio Murashige y Skoog, suplementado con distintos reguladores de crecimiento. Se cuantificó el porcentaje de inducción callogénica y caulogénica. Los brotes regenerados fueron micropropagados en medio suplementado con 2 mg L-1de BA y 0,1 mg L-1de ANA y el enraizamiento fue ensayado bajo condiciones in vitroy ex vitro. El porcentaje más alto (100%) de callogénesis en ápices meristemáticos se cuantificó en presencia de 0,5 y 1 mg L-1de 2,4-D y 3 mg L-1de ANA y en discos caulinares en presencia de 0,5 mg L-1de 2,4-D con 12.5%. Durante la caulogénesis, el promedio más alto de brotes/explante (1,1) se cuantificó en ápices meristemáticos en presencia de 5 mg L-1de ANA; mientras que en discos caulinares se observó (1,3 brotes/explante), en presencia de 0,25 mg L-1de 2,4-D. Después del segundo ciclo de micropropagación el promedio de brotes/explante desarrollados aumentó a 8,8. El mayor porcentaje (75,3%) de brotes enraizados se cuantificó en condiciones in vitro. En conclusión, elexplante primario más aconsejablepara iniciar procesos organogénicos en A. verafue ápices meristemáticos cultivados en medio con 5,0 mg L-1de ANA. Universidad Pedagógica y Tecnológica de Colombia 2019-07-23 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/9809 10.19053/01217488.v10.n2.2019.9809 Ciencia En Desarrollo; Vol. 10 No. 2 (2019): Vol 10, Núm. 2 (2019): Julio - Diciembre; 9 - 21 Ciencia en Desarrollo; Vol. 10 Núm. 2 (2019): Vol 10, Núm. 2 (2019): Julio - Diciembre; 9 - 21 2462-7658 0121-7488 spa https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/9809/8652 |
| spellingShingle | callos, cultivo in vitro, reguladores de crecimiento callus, in vitro culture, growth regulators Albarrán Ruiz, Yuri Pacheco Maldonado, José Constantino Rache Cardenal, Leidy Yanira Organogenesis in meristems and cauline disc of Aloe vera L. |
| title | Organogenesis in meristems and cauline disc of Aloe vera L. |
| title_alt | Organogénesis a partir de ápices meristemáticos y discos caulinares de Aloe vera L. |
| title_full | Organogenesis in meristems and cauline disc of Aloe vera L. |
| title_fullStr | Organogenesis in meristems and cauline disc of Aloe vera L. |
| title_full_unstemmed | Organogenesis in meristems and cauline disc of Aloe vera L. |
| title_short | Organogenesis in meristems and cauline disc of Aloe vera L. |
| title_sort | organogenesis in meristems and cauline disc of aloe vera l |
| topic | callos, cultivo in vitro, reguladores de crecimiento callus, in vitro culture, growth regulators |
| topic_facet | callos, cultivo in vitro, reguladores de crecimiento callus, in vitro culture, growth regulators |
| url | https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/9809 |
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