Electrospun poly(ɛ-caprolactone)/collagen scaffolds with potential use for skin regeneration
Current treatments for skin lesions repair are autografts, allografts and biosynthetic dermal substitutes. However, only autograft therapy gives best results, but it is subject to skin area affected in burned patient, other treatments only give temporary coverage to wound, evidencing need to generat...
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| Format: | Online |
| Language: | spa |
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Universidad Pedagógica y Tecnológica de Colombia
2019
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| Online Access: | https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/9841 |
| _version_ | 1801706345891627008 |
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| author | Lizarazo-Fonseca, Liliana Muñoz Prieto, Efrén Vera Graziano, R Camacho, Bernardo Salguero, Gustavo Silva Cote, Ingrid |
| author_facet | Lizarazo-Fonseca, Liliana Muñoz Prieto, Efrén Vera Graziano, R Camacho, Bernardo Salguero, Gustavo Silva Cote, Ingrid |
| author_sort | Lizarazo-Fonseca, Liliana |
| collection | OJS |
| description | Current treatments for skin lesions repair are autografts, allografts and biosynthetic dermal substitutes. However, only autograft therapy gives best results, but it is subject to skin area affected in burned patient, other treatments only give temporary coverage to wound, evidencing need to generate structures that besides protecting wound have biological functions that contribute to the repair or regeneration of the skin. In order to generate a dressing that meets these characteristics, poly (ℇ-caprolactone) / collagen type I scaffolds were fabricated by electrospinning to be evaluated as possible dermal substitutes. These scaffolds were characterized by SEM, contact angle, ATR-FTIR, TGA, DSC and their biocompatibility were evaluated by adhesion and proliferation of Wharton’s Jelly mesenchymal stromal cells (WJ-MSC). It was found that collagen presence in scaffolds decreases fiber diameter and improves hydrophilicity favoring adhesion of WJ-MSC. It was found that the presence of collagen in the scaffolds decreases the fiber diameter and improves the hydrophilicity favoring the adhesion processes of the CEM-GW. In addition, it was shown that it is not necessary to use high amounts of collagen to obtain a scaffold with favorable physicochemical and biological characteristics. |
| format | Online |
| id | oai:oai.revistas.uptc.edu.co:article-9841 |
| institution | Revista Ciencia en Desarrollo |
| language | spa |
| publishDate | 2019 |
| publisher | Universidad Pedagógica y Tecnológica de Colombia |
| record_format | ojs |
| spelling | oai:oai.revistas.uptc.edu.co:article-98412020-11-11T02:06:21Z Electrospun poly(ɛ-caprolactone)/collagen scaffolds with potential use for skin regeneration Andamios eletrohilados de poli(ɛ-caprolactona) /colágeno con uso potencial en regeneración de tejido cutáneo Lizarazo-Fonseca, Liliana Muñoz Prieto, Efrén Vera Graziano, R Camacho, Bernardo Salguero, Gustavo Silva Cote, Ingrid Sustitutos dérmicos, CEM-GW, lesiones cutáneas, biocompatibilidad. Dermal substitutes, CEM-GW, tissue regeneration, biocompatibility and hydrophilicity. Current treatments for skin lesions repair are autografts, allografts and biosynthetic dermal substitutes. However, only autograft therapy gives best results, but it is subject to skin area affected in burned patient, other treatments only give temporary coverage to wound, evidencing need to generate structures that besides protecting wound have biological functions that contribute to the repair or regeneration of the skin. In order to generate a dressing that meets these characteristics, poly (ℇ-caprolactone) / collagen type I scaffolds were fabricated by electrospinning to be evaluated as possible dermal substitutes. These scaffolds were characterized by SEM, contact angle, ATR-FTIR, TGA, DSC and their biocompatibility were evaluated by adhesion and proliferation of Wharton’s Jelly mesenchymal stromal cells (WJ-MSC). It was found that collagen presence in scaffolds decreases fiber diameter and improves hydrophilicity favoring adhesion of WJ-MSC. It was found that the presence of collagen in the scaffolds decreases the fiber diameter and improves the hydrophilicity favoring the adhesion processes of the CEM-GW. In addition, it was shown that it is not necessary to use high amounts of collagen to obtain a scaffold with favorable physicochemical and biological characteristics. Dentro de los tratamientos actuales para la reparación de las lesiones en piel se encuentran los autoinjertos, los aloinjertos ylos sustitutos dérmicos biosintéticos. Sin embargo, solo la terapia con autoinjerto es la que da mejores resultados y está sujeta al área de piel afectada en el paciente quemado, los demás tratamientos solo dan cobertura temporal a la herida, evidenciando la necesidad de generar estructuras que además de proteger la herida posean funciones biológicas que contribuyan a los procesos de reparación o regeneración de la piel. Con el fin de generar un apósito que reúna estas características, se fabricaron andamios de poli(ℇ-caprolactona) /Colágeno tipo I por electrohilado como posibles sustitutos dérmicos. Estos andamios se caracterizaron porSEM, ángulo de contacto, ATR-FTIR, TGA, DSC y se determinó su biocompatibilidad a partir de laadhesión y proliferación decélulas estromales mesenquimales de gelatina de Wharton (CEM-GW). Se encontró que la presencia de colágeno en los andamios disminuye el diámetro de fibra y mejora la hidrofilicidad favoreciendo los procesos de adhesión de las CEM-GW. Además, se demostró que no es necesario utilizar altas cantidades de colágeno para obtener un andamio con características fisicoquímicas y biológicas favorables. Universidad Pedagógica y Tecnológica de Colombia 2019-07-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/9841 10.19053/01217488.v10.n2.2019.9841 Ciencia En Desarrollo; Vol. 10 No. 2 (2019): Vol 10, Núm. 2 (2019): Julio - Diciembre; 197-208 Ciencia en Desarrollo; Vol. 10 Núm. 2 (2019): Vol 10, Núm. 2 (2019): Julio - Diciembre; 197-208 2462-7658 0121-7488 spa https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/9841/8663 |
| spellingShingle | Sustitutos dérmicos, CEM-GW, lesiones cutáneas, biocompatibilidad. Dermal substitutes, CEM-GW, tissue regeneration, biocompatibility and hydrophilicity. Lizarazo-Fonseca, Liliana Muñoz Prieto, Efrén Vera Graziano, R Camacho, Bernardo Salguero, Gustavo Silva Cote, Ingrid Electrospun poly(ɛ-caprolactone)/collagen scaffolds with potential use for skin regeneration |
| title | Electrospun poly(ɛ-caprolactone)/collagen scaffolds with potential use for skin regeneration |
| title_alt | Andamios eletrohilados de poli(ɛ-caprolactona) /colágeno con uso potencial en regeneración de tejido cutáneo |
| title_full | Electrospun poly(ɛ-caprolactone)/collagen scaffolds with potential use for skin regeneration |
| title_fullStr | Electrospun poly(ɛ-caprolactone)/collagen scaffolds with potential use for skin regeneration |
| title_full_unstemmed | Electrospun poly(ɛ-caprolactone)/collagen scaffolds with potential use for skin regeneration |
| title_short | Electrospun poly(ɛ-caprolactone)/collagen scaffolds with potential use for skin regeneration |
| title_sort | electrospun poly e caprolactone collagen scaffolds with potential use for skin regeneration |
| topic | Sustitutos dérmicos, CEM-GW, lesiones cutáneas, biocompatibilidad. Dermal substitutes, CEM-GW, tissue regeneration, biocompatibility and hydrophilicity. |
| topic_facet | Sustitutos dérmicos, CEM-GW, lesiones cutáneas, biocompatibilidad. Dermal substitutes, CEM-GW, tissue regeneration, biocompatibility and hydrophilicity. |
| url | https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/9841 |
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