Preliminary phospholipase, Hemolytic and Bactericidal activity of the venom of the Snake of Cascabel Tolima
In the department of Tolima, there are no studies that allow us to specify with certainty the magnitude of the ophidic accident caused by Crotalus durissus; Therefore, there is a need to generate toxicological information, as well as knowledge of the potential antimicrobial potential of molecules su...
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| Format: | Online |
| Language: | spa |
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Universidad Pedagógica y Tecnológica de Colombia
2020
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| Online Access: | https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/9869 |
| _version_ | 1801706346024796160 |
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| author | Solano Godoy, Jennifer Alexandra Molano Cardona, Emerson David Bernal Bautista, Manuel Hernando Murillo Arango, Walter |
| author_facet | Solano Godoy, Jennifer Alexandra Molano Cardona, Emerson David Bernal Bautista, Manuel Hernando Murillo Arango, Walter |
| author_sort | Solano Godoy, Jennifer Alexandra |
| collection | OJS |
| description | In the department of Tolima, there are no studies that allow us to specify with certainty the magnitude of the ophidic accident caused by Crotalus durissus; Therefore, there is a need to generate toxicological information, as well as knowledge of the potential antimicrobial potential of molecules such as proteins and peptides that make up the venom. In this work, we analyzed the electrophoretic profile by SDS-PAGE of crude venom extracted from individuals collected in the municipality of Natagaima (Tolima) and the association of its components with direct and indirect hemolytic activity on blood agar and its bactericidal activity. The potential of the crude venom against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeuroginosa was evaluated. The venom showed molecular weight bands 26.6 kDa., 17, 14.2, 6.5, 3.5 and 1.06 kDa., corresponding to reports of the venom of this species. The direct and indirect hemolytic activity was dose-dependent, with a minimum hemolytic dose of 200 mg / ml. The antimicrobial effect of the crude venom was differential on the microorganisms evaluated, presenting moderate activity on E. coli. The results constitute valuable data to preliminarily recognize the antimicrobial potential of Crotalus durissus venom (rattle) from the Natagaima-Tolima area. |
| format | Online |
| id | oai:oai.revistas.uptc.edu.co:article-9869 |
| institution | Revista Ciencia en Desarrollo |
| language | spa |
| publishDate | 2020 |
| publisher | Universidad Pedagógica y Tecnológica de Colombia |
| record_format | ojs |
| spelling | oai:oai.revistas.uptc.edu.co:article-98692021-02-03T17:14:11Z Preliminary phospholipase, Hemolytic and Bactericidal activity of the venom of the Snake of Cascabel Tolima Actividad Fosfolipasa, Hemolítica y Bactericida preliminar del Veneno de la Serpiente de cascabel del Tolima Solano Godoy, Jennifer Alexandra Molano Cardona, Emerson David Bernal Bautista, Manuel Hernando Murillo Arango, Walter Actividad bactericida Cascabel Crotalus durissus Fosfolipasas Hemolisis Toxinologia Veneno Crotalus durissus, Bactericidal activity, Hemolysis, Phospholipases, Rattlesnake, Toxinology, Venom. Bactericidal activity Rattlesnake Crotalus durissus Phospholipases Hemolysis Toxinology Venom In the department of Tolima, there are no studies that allow us to specify with certainty the magnitude of the ophidic accident caused by Crotalus durissus; Therefore, there is a need to generate toxicological information, as well as knowledge of the potential antimicrobial potential of molecules such as proteins and peptides that make up the venom. In this work, we analyzed the electrophoretic profile by SDS-PAGE of crude venom extracted from individuals collected in the municipality of Natagaima (Tolima) and the association of its components with direct and indirect hemolytic activity on blood agar and its bactericidal activity. The potential of the crude venom against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeuroginosa was evaluated. The venom showed molecular weight bands 26.6 kDa., 17, 14.2, 6.5, 3.5 and 1.06 kDa., corresponding to reports of the venom of this species. The direct and indirect hemolytic activity was dose-dependent, with a minimum hemolytic dose of 200 mg / ml. The antimicrobial effect of the crude venom was differential on the microorganisms evaluated, presenting moderate activity on E. coli. The results constitute valuable data to preliminarily recognize the antimicrobial potential of Crotalus durissus venom (rattle) from the Natagaima-Tolima area. En el departamento del Tolima no hay estudios que permitan precisar con certeza la magnitud del accidente ofídico causado por Crotalus durissus, existiendo la necesidad de generar información sobre el perfil proteico, como forma de aproximación a la compresión de algunas actividades biológicas relacionadas con la toxicidad del veneno, así como su potencial biotecnológico. En este trabajo se analizó el perfil electroforético por SDS-PAGE del veneno crudo extraído de individuos colectados en el municipio de Natagaima (Tolima) y la asociación con actividades fosfolipasa, hemolítica directa e indirecta y bactericida sobre Escherichia coli, Staphylococcus aureus y Pseudomona aeuroginosa. El veneno crudo presentó bandas de peso molecular 26.6 kDa., 17, 14.2, 6.5, 3.5 y 1.06 kDa., correspondientes con otros reportes previos del veneno para la especie. Se presentaron niveles considerables de actividades hemolítica (200 µg) y fosfolipasa (1.25 UA/mg. ± 0.88) dependientes de Calcio, y el efecto bactericida del veneno crudo fue diferencial sobre los microorganismos evaluados, presentando actividad moderada sobre Escherichia coli. Los resultados constituyen datos valiosos que confieren un acercamiento hacia el conocimiento del potencial tóxico del veneno de Crotalus durissus (cascabel) de la zonade Natagaima-Tolima, así como de la capacidad bactericida y posibles aplicaciones futuras en campos de investigación relacionados con la búsqueda de nuevos agentes antimicrobianos. Universidad Pedagógica y Tecnológica de Colombia 2020-01-25 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/9869 10.19053/01217488.v11.n1.2020.9869 Ciencia En Desarrollo; Vol. 11 No. 1 (2020): Vol 11, Núm.1 (2020): Enero-Junio; 119-127 Ciencia en Desarrollo; Vol. 11 Núm. 1 (2020): Vol 11, Núm.1 (2020): Enero-Junio; 119-127 2462-7658 0121-7488 spa https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/9869/9637 Derechos de autor 2020 CIENCIA EN DESARROLLO |
| spellingShingle | Actividad bactericida Cascabel Crotalus durissus Fosfolipasas Hemolisis Toxinologia Veneno Crotalus durissus, Bactericidal activity, Hemolysis, Phospholipases, Rattlesnake, Toxinology, Venom. Bactericidal activity Rattlesnake Crotalus durissus Phospholipases Hemolysis Toxinology Venom Solano Godoy, Jennifer Alexandra Molano Cardona, Emerson David Bernal Bautista, Manuel Hernando Murillo Arango, Walter Preliminary phospholipase, Hemolytic and Bactericidal activity of the venom of the Snake of Cascabel Tolima |
| title | Preliminary phospholipase, Hemolytic and Bactericidal activity of the venom of the Snake of Cascabel Tolima |
| title_alt | Actividad Fosfolipasa, Hemolítica y Bactericida preliminar del Veneno de la Serpiente de cascabel del Tolima |
| title_full | Preliminary phospholipase, Hemolytic and Bactericidal activity of the venom of the Snake of Cascabel Tolima |
| title_fullStr | Preliminary phospholipase, Hemolytic and Bactericidal activity of the venom of the Snake of Cascabel Tolima |
| title_full_unstemmed | Preliminary phospholipase, Hemolytic and Bactericidal activity of the venom of the Snake of Cascabel Tolima |
| title_short | Preliminary phospholipase, Hemolytic and Bactericidal activity of the venom of the Snake of Cascabel Tolima |
| title_sort | preliminary phospholipase hemolytic and bactericidal activity of the venom of the snake of cascabel tolima |
| topic | Actividad bactericida Cascabel Crotalus durissus Fosfolipasas Hemolisis Toxinologia Veneno Crotalus durissus, Bactericidal activity, Hemolysis, Phospholipases, Rattlesnake, Toxinology, Venom. Bactericidal activity Rattlesnake Crotalus durissus Phospholipases Hemolysis Toxinology Venom |
| topic_facet | Actividad bactericida Cascabel Crotalus durissus Fosfolipasas Hemolisis Toxinologia Veneno Crotalus durissus, Bactericidal activity, Hemolysis, Phospholipases, Rattlesnake, Toxinology, Venom. Bactericidal activity Rattlesnake Crotalus durissus Phospholipases Hemolysis Toxinology Venom |
| url | https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/9869 |
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