Quantitative PCR for detection of Citrus tristeza virus in Colombia

Real-time quantitative PCR (qRT-PCR) was applied using SYBR Green for the specific detection of Citrus tristeza virus (CTV) in Colombian. Genomic RNA (gRNA) was amplified using primers designed from conserved sequences in the open reading frames (ORF’s) 1b and 2. We obtained a 186 bp product with ne...

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Auteurs principaux:	Solano-Luna, Luis Miguel, Chavarro-Mesa, Edisson, Ángel-Diaz, Jorge Evelio
Format:	Online
Langue:	spa
Publié:	Universidad Pedagógica y Tecnológica de Colombia 2018
Sujets:	absolute quantification citrus diseases Closterovirus detection of plant pathogens real-Time PCR SYBR Green cuantificación absoluta detección de patógenos vegetales enfermedades de los cítricos PCR en tiempo real SYBR Green
Accès en ligne:	https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/7789